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Team:HokkaidoU Japan/Notebook/asB0034/Ptet B0034 32 asB0034 assay
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R0040-B0034-E1010-B0015 (on pSB6A1) | R0040-B0034-E1010-B0015 (on pSB6A1) | ||
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R0040-B0032-E1010-B0015 (on pSB6A1) | R0040-B0032-E1010-B0015 (on pSB6A1) | ||
| - | Ptrc-stem-asB0034-stem(on pHN1257) | + | </div> |
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| + | Ptrc-stem-asB0034-stem(on pHN1257) | ||
| + | </div> | ||
| + | <div class="step-recipe"> | ||
| + | R0040-B0032-E1010-B0015 (on pSB6A1) | ||
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| + | <div class="step-recipe"> | ||
Turbidity was controlled 0.1 | Turbidity was controlled 0.1 | ||
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| - | M9ZB 2 mL IPTG 20 µ or not for 9 hours, 11 hours, 13hours | + | M9ZB 2 mL IPTG 20 µ or not for 9 hours, |
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| + | 11 hours, 13hours | ||
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Revision as of 03:58, 18 October 2014
Notebook
Lab Documents
Ptet-B0034,B0032,-mRFP-dT
Ptet-B0032-mRFP-dT&asB0034Assay
-
Start
-
Preculture
R0040-B0034-E1010-B0015 (on pSB6A1)R0040-B0032-E1010-B0015 (on pSB6A1)Ptrc-stem-asB0034-stem(on pHN1257)R0040-B0032-E1010-B0015 (on pSB6A1)Turbidity was controlled 0.1 -
Culture
M9ZB 2 mL IPTG 20 µ or not for 9 hours,11 hours, 13hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
