Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay

From 2014.igem.org

Revision as of 02:49, 18 October 2014 by Tamaro (Talk | contribs)

Notebook
Lab Documents

140910 asB0034&B0034 mRFP Assay

  • Start

  • Double Transformation

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)
    each of 2.5µL to DH5α
  • Colony PCR

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃
  • Preculture

    Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1)
    LB 2 mL
    for 3hours
    Turbidity was controlled 0.1
  • Culture

    LB 2 mL
    IPTG 20 µ or not
    for 11 hours, 13hours, 15hours, 17 hours
  • Assay

    fluoresence imageing 514 nm 610 filter
  • Complete!