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Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 GFP Assay
From 2014.igem.org
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Revision as of 00:20, 18 October 2014
Notebook
Lab Documents
140910 asB0034 & B0034 GFP Assay
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Start
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Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)each of 2.5µL DNA to DH5α -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)LB 2 mL for 9hours Turbidity was controlled 0.1 -
Culture
M9ZB 2 mL IPTG 20 µ or notfor 9 hours, 11 hours, 13hours -
Assay
fluorescence imaging 488 nm 530 filter -
Complete!
