Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction

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Revision as of 09:47, 14 October 2014 by Osamu (Talk | contribs)

Stem-Downstream Correction

  • Start

  • Sequencing

    Plac-stem-as-stem-dT on 1A3, found stem-dn not inserted!

  • PCR

    Plac-stem-as-dT on 1A3 with XhoI-RBS(reverse) & XhoI-dT(forward)

  • Digestion

    XhoI-dT-1A3-Plac-stem-as-XhoI with XhoI

  • Primer Annealing

    stem-downstream-f & stem-downstream-r

  • Ligation

    XhoI-dT-1A3-Plac-stem-as-XhoI & stem-dn

  • Transformation

    Plac-stem-as-stem-dT on 1A3

  • Colony PCR Sat Oct 4

    Plac-stem-as-stem-dT on 1A3

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/H_Stem/Downstream_Correction"