Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction

From 2014.igem.org

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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-stem-dT on 1A3, found stem-dn not inserted!
+
R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted!
 +
 
</div>
</div>
    </div>
    </div>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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Plac-stem-as-dT on 1A3 with XhoI-RBS(reverse) & XhoI-dT(forward)
+
R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9&#8451; & XhoI_RBS_R Tm:68.2&#8451;
 +
 
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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XhoI-dT-1A3-Plac-stem-as-XhoI with XhoI
+
XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI
</div>
</div>
    </div>
    </div>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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stem_downstream_F & stem_downstream_R
+
stem_downstream_F Tm:&#8451; & stem_downstream_R
 +
 
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    </div>
    </div>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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XhoI-dT-1A3-Plac-stem-as-XhoI & stem-dn
+
XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn
</div>
</div>
    </div>
    </div>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Plac-stem-as-stem-dT on 1A3
+
R0010-stem-as-stem-B0015(on pSB1A3)
</div>
</div>
    </div>
    </div>
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  <div class="step-tile">
  <div class="step-tile">
  <h2 class="step-title">
  <h2 class="step-title">
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Colony PCR Sat Oct 4
+
Colony PCR
</h2>
</h2>
  </div>
  </div>
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        <li>
        <li>
  <time>
  <time>
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Plac-stem-as-stem-dT on 1A3
+
Sat Oct 4
</time>
</time>
</li>
</li>
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Plac-stem-as-stem-dT on 1A3
+
R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0&#8451; & 100_up_R Tm:62.6&#8451;
 +
 
</div>
</div>
    </div>
    </div>

Revision as of 01:42, 18 October 2014

Notebook
Lab Documents

Stem-Downstream Correction

  • Start

  • Sequencing

    R0010-stem-as-stem-B0015(on pSB1A3) found stem-dn not inserted!

  • PCR

    R0010-stem-as-B0015(on pSB1A3) with NheI_dT_F Tm:67.9℃ & XhoI_RBS_R Tm:68.2℃

  • Digestion

    XhoI-B0015-1A3-R0010-stem-as-XhoI with XhoI

  • Primer Annealing

    stem_downstream_F Tm:℃ & stem_downstream_R

  • Ligation

    XhoI-B0015-1A3-R0010-stem-as-XhoI & stem-dn

  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)

  • Colony PCR

    R0010-stem-as-stem-B0015(on pSB1A3) with NcoI_mRFP_F Tm:76.0℃ & 100_up_R Tm:62.6℃

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/H_Stem/Downstream_Correction"