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Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction
From 2014.igem.org
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<ul> | <ul> | ||
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Length Variation</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Length Variation</a></li> | ||
| - | <li class="ldd_contents"><a href="">Overview</a></li> | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length">Overview</a></li> |
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Method">Method</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Method">Method</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Results">Results</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Length#Results">Results</a></li> | ||
Revision as of 21:33, 17 October 2014
Notebook
Lab Documents
Stem-Downstream Correction
-
Start
-
Sequencing
Plac-stem-as-stem-dT on 1A3, found stem-dn not inserted! -
PCR
Plac-stem-as-dT on 1A3 with XhoI-RBS(reverse) & XhoI-dT(forward) -
Digestion
XhoI-dT-1A3-Plac-stem-as-XhoI with XhoI -
Primer Annealing
stem_downstream_F & stem_downstream_R -
Ligation
XhoI-dT-1A3-Plac-stem-as-XhoI & stem-dn -
Transformation
Plac-stem-as-stem-dT on 1A3 -
Colony PCR Sat Oct 4
Plac-stem-as-stem-dT on 1A3 -
Complete!
