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Team:HokkaidoU Japan/Notebook/H Stem/Downstream Correction
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Revision as of 09:46, 14 October 2014
Stem-Downstream Correction
-
Start
-
Sequencing
Plac-stem-as-stem-dT on 1A3, found stem-dn not inserted! -
PCR
Plac-stem-as-dT on 1A3 with XhoI-RBS(reverse) & XhoI-dT(forward) -
Digestion
XhoI-dT-1A3-Plac-stem-as-XhoI with XhoI -
Primer Annealing
stem-downstream-f & stem-downstream-r -
Ligation
XhoI-dT-1A3-Plac-stem-as-XhoI & stem-dn -
Transformation
Plac-stem-as-stem-dT on 1A3 -
Colony PCR Sat Oct 4
Plac-stem-as-stem-dT on 1A3 -
Complete!
