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Team:Heidelberg
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<div class="container"> | <div class="container"> | ||
<a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a> | <a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a> | ||
| - | + | <img id="ring" style="visibility:hidden;" src="/wiki/images/0/0d/Heidelberg_Firering_red.png" alt="Ring of fire Image"/> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide epic-background" style="position:relative;"> | |
| - | + | <div id="img-placeholder" class="col-lg-6 col-md-6 col-sm-12"> | |
| - | + | <img id="placeholder" class="img-responsive" src="/wiki/images/0/0d/Heidelberg_Firering_red.png" alt="Ring of fire Image"/> | |
| - | + | </div> | |
| - | + | <div class="col-lg-6 col-md-6 col-sm-12 impressiv"> | |
| - | + | <div class="hidden-sm hidden-xs" style="padding-top:100px;"></div> | |
| - | + | <h3>iGEM TEAM <span>HEIDELBERG</span> 2014</h3> | |
| - | + | <h1>THE RING<br />OF <span>FIRE</span></h1> | |
| - | + | <p>Welcome to the proteins of tomorrow.<br/>This is the iGEM team Heidelberg‘s wiki page.</p> | |
<p> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract. | <p> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract. | ||
<br/> | <br/> | ||
Scroll down to explore our project. | Scroll down to explore our project. | ||
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | <div class="arrow arrow-right"></div > | |
| - | + | <div id="scrollNag" class="arrow arrow-right"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div id="second" class="jumbotron slide grey second"> | |
| - | + | <div id="placeholder-2" class="col-lg-3 col-md-3 hidden-sm hidden-xs" > | |
| - | + | </div> | |
| - | + | <div class="col-lg-9 col-md-9 col-sm-12" style="text-align: right;"> | |
| - | + | <h1 style="text-align:right;"><span class="red-text" style="font-size:1.3em;">PLACEHOLDER</span></h1> | |
| - | + | <p><span class="red-text" style="font-size: 1.5em;">Nature</span> has made many curious inventions. One of these are circular proteins, which are conventional peptides that neither have a beginning, nor an ending</p> | |
| - | + | <p>Apart from other features, these proteins are extremely stable against | |
high temperatures, pH and proteases.</p> | high temperatures, pH and proteases.</p> | ||
| - | + | <p style="font-weight:bold;">We seeked to apply this principle of circularization in Synthetic Biology and create a way of rendering any protein heatstable.</p> | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide red red-ring"> | |
| - | + | <h2 style="text-align:center; font-weight: bold; margin-bottom:0;">Let us take you to the next level of bioengineering ...</h2> | |
| - | + | <h1 style="text-align: center; margin-top:0; font-size:3em; margin-bottom: 15px;"><span class="light-red-text">USING THE</span> Mechanism <span class="light-red-text">OF</span> SPLIT-INTEINS</h1> | |
| - | + | <div class="col-lg-6"> | |
| - | + | <p>Intein splicing is a natural process that excises one part of a protein and leaves the remaining parts irreersibly attached.</p> | |
| - | + | <p>When attaching split inteins to the ends of a normal protein, the splicing reaction connects the beginning to the ending and forms a circular protein.</p> | |
| - | + | </div> | |
| - | + | <div class="col-lg-6"> | |
| - | + | Placeholder | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-right"></div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide epic-background" style="color:white;"> | |
| - | + | <div class="col-lg-4 col-md-4 col-xs-12" style="height: 410px; position: relative"> | |
| - | + | <div id="circ-box" class="descr-box"> | |
| - | + | <a href="/Team:Heidelberg/Toolbox/Circularization"> | |
| - | + | <img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon"> CIRCULARIZATION | |
| - | + | </a> | |
| - | + | </div> | |
| - | + | <div id="oligo-box" class="descr-box"> | |
| - | + | <a href="/Team:Heidelberg/Toolbox/Oligomerization"> | |
| - | + | <img src="/wiki/images/4/40/Oligomerization.png" id="oligo-icon" class="toolbox-icon"> OLIGOMERIZATION | |
| - | + | </a> | |
| - | + | </div> | |
| - | + | <div id="fusion-box" class="descr-box"> | |
| - | + | <a href="/Team:Heidelberg/Toolbox/Fusion"> | |
| - | + | <img src="/wiki/images/8/87/Heidelberg_Toolbox_Fusion.png" id="fusion-icon" class="toolbox-icon"> FUSION | |
| - | + | </a> | |
| - | + | </div> | |
| - | + | <div id="onoff-box" class="descr-box"> | |
| - | + | <a href="/Team:Heidelberg/Toolbox/OnOff"> | |
| - | + | <img src="/wiki/images/c/c2/Heidelberg_Toolbox_On-Off.png" id="onoff-icon" class="toolbox-icon"> ON/OFF | |
| - | + | </a> | |
| - | + | </div> | |
| - | + | <div id="purification-box" class="descr-box"> | |
| - | + | <a href="/Team:Heidelberg/Toolbox/Purification"> | |
| - | + | <img src="/wiki/images/0/04/Heidelberg_Toolbox_Purification.png" id="purification-icon" class="toolbox-icon"> PURIFICATION | |
| - | + | </a> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8 col-md-8 col-xs-12"> | |
| - | + | <h1 style="text-align: right; font-size: 3em;" >... and show you the WORLD of <br /> | |
| - | + | <span class="red-text">post-translational MODIFCATION</span> | |
| - | + | </h1> | |
| - | + | <p>The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.</p> | |
| - | + | <p>Our toolbox contains several tools which are supergeil. Here you can find out more about our Toolbox.</p> | |
| - | + | <p>In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.</p> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div style="position:relative;" class="row"> | |
| - | + | <div class="jumbotron slide grey"> | |
| - | + | <div> | |
| - | + | <div class="col-lg-4 col-lg-push-8" style="z-index:5;"> | |
| - | + | <img class="img-responsive" src="/wiki/images/a/a6/Heidelberg_Project_Dnmt1.png" /> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8 col-lg-pull-4"> | |
| - | + | <h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1> | |
| - | + | <p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p> | |
| - | + | <p>The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. | |
| - | + | For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization. | |
| - | + | </p> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | <div class="title-wrapper-dnmt1"> | |
| - | + | <span class="title-dnmt1">PCR 2.0</span> | |
| - | + | <span class="special-span-dnmt1"></span> | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div style="position:relative;" class="row"> | |
| - | + | <div class="jumbotron slide red"> | |
| - | + | <div> | |
| - | + | <div class="col-lg-4" style="z-index:5;"> | |
| - | + | <img style="margin-top:25px;"class="img-responsive" src="/wiki/images/8/82/Heidelberg_Project_Xylanase.png" /> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8"> | |
| - | + | <h1 style="text-align: right; color: black; padding-right: 130px;" ><span style="font-size: 0.8em;">circular <span style="color:white;">heat-stable</span></span><br><span style="font-size:1.3em;">Xylanase</span></h1> | |
| - | + | <p>Xylanase is an important enzyme for the pulp and paper industry.</p> | |
| - | + | <p>Bla bla</p> | |
| - | + | <p>In future Xylanase could be used for the production of biofuel.</p> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | <div class="title-wrapper-xylanase" style="text-align: right; color:white;"> | |
| - | + | <span class="title-xylanase">INDUSTRY</span> | |
| - | + | <span class="special-span-xylanase"></span> | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide dark-grey" style="color:white;"> | |
| - | + | <div> | |
| - | + | <div class="col-lg-4 col-lg-push-8" style="z-index:5;"> | |
| - | + | <img class="img-responsive" src="/wiki/images/d/df/Heidelberg_Lysozyme.png" /> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8 col-lg-pull-4"> | |
| - | + | <h1 style="text-align: left;" ><span style="font-size:1.3em;">LINK it!</span></h1> | |
| - | + | <p>Could every protein becomes heat stable by circularization, even if it´s the most complex of all?</p> | |
| - | + | <p>Circularization is a narrow path between gaining heat-stability and loosing function due to deformation. We developed a linker software, which predict the perfect linker depending on the folding structure of every protein.</p> | |
| - | + | </div> | |
| - | + | <div class="col-lg-12"> | |
| - | + | <p>In an extensive linker screening our software was improved and calibrated using the lambda phage lysozyme.</p> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide grey"> | |
| - | + | <div> | |
| - | + | <div> | |
| - | + | <div class="col-lg-3" style="z-index:5;"> | |
| - | + | <img class="img-responsive" src="/wiki/images/e/e5/Heidelberg_Frontpage_igemathome.png" /> | |
| - | + | </div> | |
| - | + | <div class="col-lg-9"> | |
| - | + | <h1 style="text-align: left; color:#DE4230;" ><span style="font-size:1.5em; font-weights:bold;">CALCULATE it!</span></h1> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8"> | |
| - | + | <p><br/>After calculating eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.</p> | |
| - | + | <p>The iGEM Team Heidelberg developed <a href="/Team:Heidelberg/Software/igemathome">iGEM@home</a>, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their are idle.</p> | |
| - | + | <p>As a new tool for the iGEM community this system enables every student team to archieve their modeling without access to big server farms.</p> | |
| - | + | </div> | |
| - | + | <div class="col-lg-4"> | |
| - | + | <img style="position:relative; top: -35px;" class="img-responsive" src="/wiki/images/5/52/Heidelberg_Frontpage_igemathome_cloud.png" /> | |
| - | + | </div> | |
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| - | + | <div class="arrow arrow-left"></div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div class="row"> | |
| - | + | <div class="jumbotron slide dark-grey" style="color:white; position:relative;"> | |
| - | + | <div class="col-lg-12"><h1 style="text-align:center;">Who are we?</h1></div> | |
| - | + | <div class="col-lg-4" style="position:absolute; bottom:0; left: 45px;"> | |
| - | + | <img class="img-responsive" src="/wiki/images/5/5e/Heidelberg_Frontpage_Team.png" /> | |
| - | + | </div> | |
| - | + | <div class="col-lg-8 col-lg-offset-4"> | |
| - | + | <p>We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.</p> | |
| - | + | <p>For our project we got great feedback and support from our supervisors.</p> | |
| - | + | <p>Take a look at our <a href="/Team:Heidelberg/Team">Teampage</a>!</p> | |
| - | + | <h1>Thank you!</h1> | |
| - | + | <p>We want thank all people who helped us and supported our work in the lab.</p> | |
| - | + | </div> | |
| - | + | ||
| - | + | <div class="clearfix"></div> | |
| - | + | </div> | |
| + | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
{{:Team:Heidelberg/Templates/IncludeJS|:Team:Heidelberg/js/frontpage}} | {{:Team:Heidelberg/Templates/IncludeJS|:Team:Heidelberg/js/frontpage}} | ||
Revision as of 14:49, 11 October 2014
iGEM TEAM HEIDELBERG 2014
THE RING
OF FIRE
Welcome to the proteins of tomorrow.
This is the iGEM team Heidelberg‘s wiki page.
Click here to view our abstract.
Scroll down to explore our project.
CIRCULARIZATION
OLIGOMERIZATION
FUSION
ON/OFF
PURIFICATION
... and show you the WORLD of
post-translational MODIFCATION
The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.
Our toolbox contains several tools which are supergeil. Here you can find out more about our Toolbox.
In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.
circular heat-stable
DNMT1
Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?
The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization.
PCR 2.0
circular heat-stable
Xylanase
Xylanase is an important enzyme for the pulp and paper industry.
Bla bla
In future Xylanase could be used for the production of biofuel.
INDUSTRY
LINK it!
Could every protein becomes heat stable by circularization, even if it´s the most complex of all?
Circularization is a narrow path between gaining heat-stability and loosing function due to deformation. We developed a linker software, which predict the perfect linker depending on the folding structure of every protein.
In an extensive linker screening our software was improved and calibrated using the lambda phage lysozyme.
CALCULATE it!
After calculating eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.
The iGEM Team Heidelberg developed iGEM@home, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their are idle.
As a new tool for the iGEM community this system enables every student team to archieve their modeling without access to big server farms.
Who are we?
We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.
For our project we got great feedback and support from our supervisors.
Take a look at our Teampage!
Thank you!
We want thank all people who helped us and supported our work in the lab.
