Team:ETH Zurich/lab/bead
From 2014.igem.org
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- | |Bacteria are resuspended and diluted in NaCl-solution (0.9 % in | + | |Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H<sub>2</sub>O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device. Droplets should fall form a height of approximately 30 cm into a CaCl<sub>2</sub> solution. |
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|[[File:ETH2014_BeadsProduction.jpg|300px]] | |[[File:ETH2014_BeadsProduction.jpg|300px]] | ||
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- | |A more detailed protocol can be found [https://2014.igem.org/Team:ETH_Zurich/lab/protocols here] | + | |A more detailed protocol can be found [https://2014.igem.org/Team:ETH_Zurich/lab/protocols here.] |
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Revision as of 16:12, 15 October 2014
Beads
Overview
Alginate beads are commonly used for immobilization of macromolecules and cells, however, they are also applied for pharmaceutical purposes such as drug delivery. The polymer
Production
Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H2O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device. Droplets should fall form a height of approximately 30 cm into a CaCl2 solution. |
A more detailed protocol can be found here. |
Loading the Chip
The beads are better visible in liquid when light shines from the side. So it's easier to load the the chip with help of a cell phone light. |
Features