Team:ETH Zurich/data

From 2014.igem.org

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(Our Favorite New Characterized Parts)
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==Our Favorite New Characterized Parts==
==Our Favorite New Characterized Parts==
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#[http://parts.igem.org/Part:BBa_K1541009 Main Page] - '''sfGFP under Riboregulated Promoter (RhlR/C4-HSL, RR12), BBa_K1541009:''' this part contains the same riboregulated promoter system as the part [http://parts.igem.org/Part:BBa_K1541009 BBa_K1541009] succeeded by the gene for sfGFP. This construct was used to characterize the properties of the riboregulated quorum sensing promoter [http://parts.igem.org/Part:BBa_K1541009 BBa_K1541009] and can be used in a C4-HSL sensor where low leakiness is desirable.<html><br><br></html>
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#[http://parts.igem.org/Part:BBa_K1541009 Main Page] - '''sfGFP under Riboregulated Promoter (RhlR/C4-HSL, RR12), BBa_K1541009:''' this riboregulated promoter construct contains the quorum sensing promoter [http://parts.igem.org/Part:BBa_R0071 BBa_R0071] which can be activated in presence of [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (BBa_C0171)] and C4-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0170 RhlI (BBa_C0170)]) succeeded by the gene for sfGFP. However, this promoter by itself shows some leakiness. Together with the ribogegulator 12<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced. This construct was used to characterize the properties of the riboregulated quorum sensing promoter [http://parts.igem.org/Part:BBa_K1541009 BBa_K1541009] and can be used in a C4-HSL sensor where low leakiness is desirable.<html><br><br></html>
#[http://parts.igem.org/Part:BBa_K1541017 Main Page] - '''''rhlI'' with optimized RBS, BBa_K1541017:''' this part is similar to the part [http://parts.igem.org/Part:BBa_I9026 BBa_I9026], but contains instead of the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] a RBS which was specifically optimized for [http://parts.igem.org/Part:BBa_C0170 ''rhlI'' (C0170)] using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>. With higher levels of RhlI, the production of the enzyme's product C4-HSL can be improved.
#[http://parts.igem.org/Part:BBa_K1541017 Main Page] - '''''rhlI'' with optimized RBS, BBa_K1541017:''' this part is similar to the part [http://parts.igem.org/Part:BBa_I9026 BBa_I9026], but contains instead of the RBS [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] a RBS which was specifically optimized for [http://parts.igem.org/Part:BBa_C0170 ''rhlI'' (C0170)] using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>. With higher levels of RhlI, the production of the enzyme's product C4-HSL can be improved.
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Revision as of 19:34, 16 October 2014

iGEM ETH Zurich 2014