Team:ETH Zurich/blog

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<html> <h4 style='color:#FFFFFF; text-align:center;'> We have uploaded our chronological progress in several articles which are classified by subject. Click on the triangles below to filter the category of articles you want to read. If you want an even more precise filtering in that category, you can choose subjects on the mosaic around.</h4> <br/> <br/> </html>
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<h1><a href="#main" id="logo" class="scrolly">Lab Notebook</a></h1>
 
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<h3><a href="#">Wednesday, July 1st</a></h3>
 
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We did ................
 
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<h3><a href="#">Wednesday, July 2nd</a></h3>
 
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We did ................
 
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<h3><a href="#">Week 1 : 9-15 June</a></h3>
 
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We did .........
 
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<h4>Tuesday, June 10th</h4>
 
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Preparation of
 
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-SOC
 
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-TFB1
 
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-TFB2 (pH was set to 5.6 instead of 5.8)
 
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-Agar plates (containing ampicillin (200 mg/L) or chloramphenicol (34 mg/L))
 
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According to qiagen DNA protocols & application
 
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Modifications:
 
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-150 ml Glycerol per 1 L (15%) are added to TFB1 and TFB2 (instead of 15 mL)
 
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-1.4 g/l CaCl2 dihydrate are used in TFB1
 
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-11.0 g/l CaCl2 dihydrate are used in TFB2
 
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-SOC is sterilized by filtration (not autoclaved)
 
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Latest revision as of 03:32, 18 October 2014

iGEM ETH Zurich 2014