Team:Duke/Notebook/Protocols

From 2014.igem.org

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<li> Making CCMB 80 Buffer
<li> Making CCMB 80 Buffer
<ul>
<ul>
-
<li>
+
<li>10 mM KOAc pH 7.0 (10 ml of a 1M stock/L)</li>
-
<li>
+
<li>80 mM CaCl2.2H2O (11.8 g/L)</li>
 +
<li>20 mM MnCl2.4H2O (4.0 g/L)</li>
 +
<li>10 mM MgCl2.6H2O (2.0 g/L)</li>
 +
<li>10% glycerol (100 ml/L)</li>
 +
<li>adjust pH DOWN to 6.4 with 0.1N HCl if necessary</li>
</ul>
</ul>
</li>
</li>

Revision as of 18:52, 14 August 2014

Chemically Competent Cells
Protocol 2
Protocol 3
Protocol 4
Protocol 5
Protocol 6
Protocol 7
Protocol 8
Protocol 9
Protocol 10
Protocol 11
Protocol 12
Protocol 13
Protocol 14
Protocol 15
Protocol 16
Protocol 17
Protocol 18
Protocol 19
Protocol 20
Protocol 21
Protocol 22
Protocol 23
Protocol 24
Protocol 25

Chemically Competent Cells

This protocol for making chemically competent E. coli for transformations. It is derived from the official iGEM protocol, which can be found here
  1. Making CCMB 80 Buffer
    • 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L)
    • 80 mM CaCl2.2H2O (11.8 g/L)
    • 20 mM MnCl2.4H2O (4.0 g/L)
    • 10 mM MgCl2.6H2O (2.0 g/L)
    • 10% glycerol (100 ml/L)
    • adjust pH DOWN to 6.4 with 0.1N HCl if necessary
  2. Culturing Cells
  3. Step 3

Protocol

This is a standard protocol for achieving this result.
  1. Step 1
  2. Step 2
  3. Step 3

Protocol

This is a standard protocol for achieving this result.
  1. Step 1
  2. Step 2
  3. Step 3

Protocol

This is a standard protocol for achieving this result.
  1. Step 1
  2. Step 2
  3. Step 3

Protocol

This is a standard protocol for achieving this result.
  1. Step 1
  2. Step 2
  3. Step 3

Retrieved from "http://2014.igem.org/Team:Duke/Notebook/Protocols"