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| - | <table padding="50px">
| + | <div class=" month"><a href=" https:// 2014.igem.org/Team: Duke/ Notebook/ Overview"> Overview of Each Month </ a></ div> |
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| + | <div class="month"><a href="https://2014.igem.org/Team:Duke/Notebook/Months">Notebook Entries by Month</a></div> |
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| + | <div class=" month"><a href=" https:// 2014. igem. org/ Team:Duke/ Notebook/ Protocols"> Protocols</ a></div> |
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| - | <div class="rightnb"> | + | |
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| - | <div class="day">
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| - | <a id="apr7"><h2> April 7 </h2></a> | + | |
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| - | <p><i>Objective: Create Z4EV-dCas9-Mxi1 construct</i></p>
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| - | <p>Matthew Farnitano and Matthew Faw</p>
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| - | <p>PCR of Z4EV from pMN10</p>
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| - | <ul>
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| - | <li>new oligos SpeI-Z4EV5p and NcoI-spc-Z4EV3p (diluted to 100uM) </li>
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| - | <li>4 tubes with 0, 0.3, 0.6, 1 uL template </li> | + | |
| - | <ul><li>iGEM 3-step protocol with 65C anneal, 20 sec extend</li></ul>
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| - | <li>Note: did not dilute template beforehand (do this in future)</li>
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| - | </ul>
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| - | <p>Results (04/08/2014): Lanes 2 and 3 produced band at ~0.7-0.8 kb (agarose gel)</p>
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| - | <ul>
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| - | <li>Expected band size 715 bp </li>
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| - | <li>Control (no template) showed no band, lane 4 produced faint band </li>
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| - | <li>Lanes 3 and 4 showed strong band at ~5-6 kb (template expected 5.1 kb) </li>
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| - | </ul>
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| - | <p>Next steps: </p>
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| - | <ul>
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| - | <li>Run on gel to confirm (4/8) </li>
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| - | <li>digest PCR product and dCas9/Mxi1 with SpeI/NcoI to ligate (4/8) </li> | + | |
| - | </ul>
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| - | </div> | + | |
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| - | <div class="day"> | + | |
| - | <h2><a id=" apr8"> April 8 </ a></ h2> | + | |
| - | Ut aliquet nunc urna, bibendum lobortis erat iaculis vitae. Etiam nisl nisi, ullamcorper eget felis et, scelerisque pulvinar massa. Suspendisse hendrerit nec nisi ac blandit. Donec euismod a neque a hendrerit. Phasellus venenatis laoreet ipsum sed pellentesque. Maecenas aliquet orci vel metus viverra, ac fermentum sapien elementum. Nulla pharetra aliquam gravida. Vivamus vitae feugiat mi. Morbi ac felis ut ante mattis tempor. Morbi fermentum vitae metus sit amet luctus. Fusce nibh turpis, adipiscing quis urna vel, porta mattis felis. Pellentesque lacinia tempor pellentesque.
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| - | <a id="apr10"> April 10 </a>
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| - | Aliquam vestibulum ornare mauris, tincidunt rhoncus orci euismod a. Maecenas mollis felis et leo varius, at blandit turpis auctor. Nam at dictum quam, sit amet vehicula ligula. Nullam faucibus neque ac libero tempor malesuada. Quisque semper turpis luctus odio condimentum rutrum. Cras sodales ultrices condimentum. Aliquam ultrices risus at diam pretium, quis bibendum lacus elementum. Duis tincidunt nunc nunc, vitae sodales orci luctus eget. Proin ante enim, facilisis vel sapien nec, dapibus suscipit sapien. Mauris aliquet sodales lobortis. In tempus neque magna, consectetur mollis turpis laoreet dignissim. Vivamus a dui turpis. Nullam libero arcu, vestibulum sed tellus ac, ornare semper elit. Quisque facilisis egestas cursus. Curabitur fermentum leo ut odio dignissim euismod. Integer pellentesque vestibulum neque, sit amet malesuada nunc sollicitudin vel.
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| - | Sed in tortor purus. Donec elit nunc, commodo vel mollis in, iaculis id nunc. Praesent ut convallis dui, a bibendum dui. Donec non malesuada quam. Duis sem urna, tincidunt sit amet odio sed, gravida tincidunt enim. Cras risus elit, laoreet vitae feugiat non, viverra vel eros. Suspendisse potenti. Maecenas vel suscipit eros.
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