Team:Cornell/project/drylab/testing
From 2014.igem.org
(Difference between revisions)
Line 46: | Line 46: | ||
<div class="col-md-8 col-xs-12"> | <div class="col-md-8 col-xs-12"> | ||
<h1>Luria Broth Testing</h1> | <h1>Luria Broth Testing</h1> | ||
- | One factor in the longevity of living cells in our fiber reactor system is the retention time of growth media in the outer chamber. To test this retention time, a test was conducted to see how long it took for LB to | + | One factor in the longevity of living cells in our fiber reactor system is the retention time of growth media in the outer chamber. To test this retention time, a test was conducted to see how long it took for LB to completely leave the fiber reactor. Tonic water fluoresces under UV light because it contains quinine, so solutions of LB and tonic water at varying ratios were run through the fiber reactor. The volume and amount of dilution of the mixture leaving the fiber reactor was measured via the amount of fluorescence. Overall, it was determined that LB does not remain in the fiber reactor for a long enough time to support growth. To support long-term growth, we would have to incorporate a separate media cycling system into the reactor. However, this reactor system is designed to work with slowly growing cells that does not require media for growth, so this is not a huge concern for our project. The picture on the right, from left to right, is 50/50 LB-tonic water mixture, the output from the fiber reactor, the mixture left in the fiber reactor, and water. |
<br><br> | <br><br> | ||
</div> | </div> |
Revision as of 03:42, 16 October 2014