Team:CSU Fort Collins

From 2014.igem.org

(Difference between revisions)
 
(47 intermediate revisions not shown)
Line 72: Line 72:
#textbox span{
#textbox span{
-
   color:#FF8533;
+
   color:#E6B800;
}
}
.photo{
.photo{
Line 154: Line 154:
}
}
#cssmenu > ul > li:hover > a {
#cssmenu > ul > li:hover > a {
-
   color: #f2a76d;
+
   color: #E6B800;
}
}
#cssmenu li.has-sub::after {
#cssmenu li.has-sub::after {
Line 170: Line 170:
}
}
#cssmenu > ul > li:hover::after {
#cssmenu > ul > li:hover::after {
-
   border-top-color: #f2a76d;
+
   border-top-color: #E6B800;
}
}
#indicatorContainer {
#indicatorContainer {
Line 261: Line 261:
}
}
#cssmenu ul ul li:hover > a {
#cssmenu ul ul li:hover > a {
-
   background: #e2e2e2;
+
   background: white;
-
   color: #FF8533;
+
   color: #E6B800;
}
}
#cssmenu ul ul li:last-child > a,
#cssmenu ul ul li:last-child > a,
Line 456: Line 456:
   margin:auto;
   margin:auto;
   vertical-align: top;
   vertical-align: top;
 +
border-bottom:1px solid #222222;
 +
color:#222222;
}
}
 +
.wrapper a{
 +
text-decoration:1px dotted #222222;
 +
}
 +
.page, .container{
.page, .container{
   width:50%;
   width:50%;
Line 471: Line 477:
}
}
.page a:hover, .container a:hover{
.page a:hover, .container a:hover{
-
   color:#FF8533;
+
   color:#E6B800;
   text-decoration:none;
   text-decoration:none;
}
}
Line 481: Line 487:
}
}
h2,h3,.toc,.show,.hide,.content{
h2,h3,.toc,.show,.hide,.content{
-
   color:#222222;
+
   color:#E6B800;
   font-family:'Bitter',serif;
   font-family:'Bitter',serif;
}
}
Line 509: Line 515:
}
}
.month-group:hover{
.month-group:hover{
-
   color:#FF8533;
+
   color:#E6B800;
}
}
.toc a, .toc2 a, #navi a{
.toc a, .toc2 a, #navi a{
Line 517: Line 523:
}
}
.toc a:hover, #ftoc>a:hover, #navi a:hover, .show:hover, .hide:hover, .container>a:hover{
.toc a:hover, #ftoc>a:hover, #navi a:hover, .show:hover, .hide:hover, .container>a:hover{
-
   color:#FF8533 !important;
+
   color:#E6B800 !important;
   cursor:pointer;
   cursor:pointer;
}
}
Line 537: Line 543:
   height:250px;
   height:250px;
   vertical-align:top;
   vertical-align:top;
-
   background-color:#222222;
+
   background-color:#E6B800;
-
   overflow: hidden
+
   overflow: hidden;
 +
}
 +
#banner a:hover{
 +
  opacity:0.7;
}
}
.fixed {
.fixed {
Line 570: Line 579:
.toc2{
.toc2{
   display:block;
   display:block;
 +
}
 +
.footer{
 +
    background-color:#222222;
 +
    width:100%;
 +
    margin:auto;
 +
    padding:15px;
 +
    color:#efefef;
 +
    font-size:14px;
 +
}
 +
.footer table{
 +
    background-color:#222222;
 +
    color:#efefef;
 +
    margin:auto;
 +
    text-align:left;
 +
  width:100%;
 +
}
 +
.footer a{
 +
    color:#efefef;
 +
}
 +
.button{
 +
background-color:#E6B800;
 +
color:#222222;
 +
border-radius:10px;
 +
font-family:'Muli',sans-serif;
 +
width:300px;
 +
text-align:center;
 +
vertical-align:center;
 +
padding:15px;
 +
margin:30px;
}
}
 +
wrapper table{
 +
width:60%;
 +
background-color:#efefef;
 +
}
 +
table{
 +
background-color:#efefef;
 +
margin:auto;
 +
}
 +
table td{
 +
text-align:center;
 +
margin:auto;
 +
width:400px;
 +
}
 +
wrapper table td{
 +
width:300px;
 +
padding:10px;
 +
overflow:hidden;
 +
}
 +
#photo{
 +
width:300px;
 +
margin:auto;
 +
padding:10px;
 +
}
</style>
</style>
Line 581: Line 642:
<body style='margin:0'>
<body style='margin:0'>
-
<div id='banner'><center><img src='http://oi57.tinypic.com/1498eg5.jpg'/>
+
<div id='banner'><center><img src='http://oi57.tinypic.com/1498eg5.jpg' style='width:100%'/><a href='https://2014.igem.org'><img src='https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png' style="width:80px; position:absolute; top: 10px; right: 20px; opacity:0.95;"/></a>
</center></div>
</center></div>
Line 595: Line 656:
       </ul>
       </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Team/'><span>Team</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Members/'><span>Team</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
-
         <li class='last'><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
 +
         <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li>
       </ul>
       </ul>
   </li>
   </li>
-
   <li class='has-sub' class='active'><a href='Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
+
   <li class='has-sub' class='active'><a href='/Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
     <ul style="display:block">
     <ul style="display:block">
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
Line 615: Line 677:
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
         <ul>
         <ul>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/'><span>Biosensor</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'><span>Biosensor</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun"><span>June</span></a></li>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li>
-
               <li><a href='#'><span>August</span></a></li>
+
               <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li>
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li>
-
              <li><a href="#"><span>July</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
+
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
Line 649: Line 707:
   </ul>
   </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/HumPrac/'><span>Human Practices</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
-
        <li><a href='/Team:CSU_Fort_Collins/Outreach/'><span>Outreach</span></a></li>
+
         <li class='last'><a href='/Team:CSU_Fort_Collins/Outreach/'><span>Outreach</span></a></li>
-
         <li class='last'><a href='/Team:CSU_Fort_Collins/Ethics'><span>Ethics Miniseries</span></a></li>
+
       </ul>
       </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Achievements/'><span>Achievements</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
Line 662: Line 719:
       </ul>
       </ul>
   </li>
   </li>
-
   <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
+
   <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
</ul>
</ul>
</div>
</div>
 +
<div class="clear"></div>
 +
<div class='wrapper'>
-
<div class="clear"></div>
+
<div class="container">
 +
    <h1>We are CSU iGEM <br> an undergraduate synthetic biology team</h1>
 +
We are a group of young researchers from Colorado State University working to build a pathway which breaks down spent frying oil in order to manufacture a value-added terpenoid.
 +
   
 +
    </div>
 +
 
 +
<div class='button'>
 +
<a href='/Team:CSU_Fort_Collins/Project/' style='font-weight:bold; color:#222222'>
 +
  Read more about our Project
 +
</a>
 +
</div>
 +
 
 +
  </center>
 +
</div>
<div class='wrapper'>
<div class='wrapper'>
-
  <center>
+
<table>
-
     <h1>Welcome to CSU Fort Collin's 2014 Wiki</h1>
+
<tr>
-
 
+
     <td><img src='https://static.igem.org/mediawiki/2014/c/c1/Team-CSU_Team2.jpg' id='photo'/><br>
-
     <div class="container">
+
        <div class='button'>
-
      <h3>Our Project</h3>
+
        <a href='/Team:CSU_Fort_Collins/Members/' style='font-weight:bold; color:#222222'>
 +
            Meet Our Team
 +
        </a>
 +
        </div>
 +
     </td>
 +
    <td ><img src='https://static.igem.org/mediawiki/2014/8/80/Team-CSU_Ntbk2.jpg' id='photo'/><br>
 +
        <div class='button'>
 +
        <a href='/Team:CSU_Fort_Collins/Notebook/' style='font-weight:bold; color:#222222'>
 +
            Read Our Notebook
 +
        </a>
 +
        </div>
 +
</span>
 +
    </td>
 +
    <td><img src='https://static.igem.org/mediawiki/2014/2/24/Team_CSU-Handshake2.jpg' id='photo'/><br>
 +
        <div class='button'>
 +
        <a href='/Team:CSU_Fort_Collins/Collab/' style='font-weight:bold; color:#222222'>
 +
            See How We Work With Others
 +
        </a>
 +
        </div>
 +
    </td>
 +
</tr>
 +
</table>
 +
</div>
-
      Approximately 3 billion gallons per year of used frying oil are produced in the U.S. alone. While some recycling efforts are put forth to turn used frying oil into biodiesel and some companies are working on more effective ways to recycle the frying oil, the iGEM team at Colorado State University is working to turn spent frying oil into a high value product. There are four major components to this year’s project including; the breakdown of frying oil, a biosensor for detecting the breakdown of frying oil, production of a high value product, and a kill switch to kill the bacteria if they were to be released into the environment. Our team aims to put each of these components into Escherichia coli. 
+
<center>
-
<br><br>
+
<div class='container'>
-
In order to detect that the process is working we have developed a biosensor that will detect the breakdown of Acyl-CoA, a byproduct of the breakdown pathway of lipids, in E. coli. Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.
+
<center>Special thanks to <a href='/Team:CSU_Fort_Collins/Sponsors/'>our sponsors</a> who made this team possible</center>
 +
</div>
 +
</center>
 +
 
 +
</div>
<br><br>
<br><br>
-
In order to break down the frying oil we will be taking advantage of the cell’s natural ability to break down fatty acids for use in the Kreb’s cycle. We will be upregulating the limiting enzymes that aid in the breakdown of fatty acids in order to have the cell produce more Acetyl-CoA.
 
-
<br><br>
 
-
This Acetyl-CoA can then be used in the mevalonate pathway. This pathway is common to most plants as well as yeast. We will be taking the genes from yeast and constructing two operons in order to produce isopentenyl pyrophosphate (IPP). While both IPP and dimethylallyl pyrophosphate (DMAPP) are both naturally produced in E. coli  through the non-mevalonate pathway , the mevalonate pathway provides a more efficient route from acetyl-CoA to terpenoid production than the alternative. Our high-value product will be a terpenoid that has yet to be determined.
 
-
<br><br>
 
-
For our kill switch, we will be using a gene named Killer Red which is a mutant of hydrozoan chromoprotein anm2CP from Anthomedusae sp. DC-2005 . Our team plans to insert this gene into our construct and by doing so, it will provide a fail-safe in the unlikely event of our E. coli escaping into the environment. The Killer Red gene has a repressible promoter in which tryptophan acts as the repressor. There will be tryptophan present while the cell is used to break down the frying oil, but is not highly present in the environment. Without the presence of tryptophan the Killer Red gene will be activated and white light will produce reactive oxygen species within the cell, causing cell death.
 
-
<br><br>
 
-
Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.
 
-
    </div>
+
 
-
  </center>
+
<div class="footer">
 +
    <center><table><center><td><p>© Colorado State University iGEM 2014 | Colorado State University, Campus Delivery 1370, Fort Collins, CO 80523-1370 | <a href="/Team:CSU_Fort_Collins/Contact/" > Contact Us </a>| <a href="/Team:CSU_Fort_Collins/Sponsors/"> Become a Sponsor </a> | <a href="http://www.facebook.com/csu.igem.2014" >Facebook</a> | <a href="http://twitter.com/CSU_iGEM" >Twitter</a></td></center></p>
 +
 
 +
</table></center>
</div>
</div>
 +
 +

Latest revision as of 22:49, 17 October 2014

CSU iGEM 2014

We are CSU iGEM
an undergraduate synthetic biology team

We are a group of young researchers from Colorado State University working to build a pathway which breaks down spent frying oil in order to manufacture a value-added terpenoid.
Read more about our Project

Meet Our Team

Read Our Notebook

See How We Work With Others
Special thanks to our sponsors who made this team possible


© Colorado State University iGEM 2014 | Colorado State University, Campus Delivery 1370, Fort Collins, CO 80523-1370 | Contact Us | Become a Sponsor | Facebook | Twitter

Retrieved from "http://2014.igem.org/Team:CSU_Fort_Collins"