1

Protocols

01 Gibson Assembly
02 Cloning Genes
03 Plasmid Miniprep
04 Yeast DNA Extraction
05 Gel Electrophoresis
06 PCR Product Purification

2

Daily Notes

Biosensor

07June: PAR, PLR, and PFL Assembly
08July: PLR, PFL, and PFL2 Assembly
09August: Scientists vs. the PCR Machine

Breakdown

10July: Lac Promoter and FadD Assembly
11August: More of the Same, But Better
12September: One Thousand and One Nights

High-Value Product

13June: pMBI Assembly
14July: pMBI and pMevT Assembly

Kill Switch

15July: TRP/KillerRed Assembly
16September: Troubleshooting and Biobrick Success