Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Aug

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August



  • Cultivation of E.coli with neutral red and bromphenol blue
    • This week we tested if neutral red and bromphenol blue have an influence on the growth of E. coli. Therefore we cultivated E.coli wildtype in M9 medium with glucose supplemented with 100 µM of neutral red or bromphenol blue. Samples were taken for OD600 measurement to document the growth curve.
  • Deletion of dcuB and integration of oprF into chromosome
    • This week we checked our first colonies to verify the deletion of dcuB
      • pR6K
        • The pR6K-cassette for the dcuB deletion was purified out of the gel
          • Transformation of pR6K-cassette for the deletion of dcuB with electrocompotetent cells
      • pRedET

  • Deletion of dcuB and integration of oprF into chromosome
    • This week we contninued to test colonies for the verification of the deletion if dcuB

  • Fum A


  • Deletion of dcuB and integration of oprF into chromosome
    • This week we tested the next colonies for the deletion of dcuB and performed the NPN-assay to verify the integration of oprF into chromosome
      • oprF
        • NPN-uptake assay for porine verification



  • ccm
    • This week we performed Eckhardt gels as a new method besides Colony PCR to identify positiv colonies
      • Eckhardt gel of ccm was performed two times
        • Bands not impeccable to identify (~6230 bp) and therefore a restriction digestion was done
          • Bands not as expected (~2070 bp and ~6239 bp)