Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Jun

From 2014.igem.org

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                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>alsS</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>alsS</i></li>
-
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>alsS</i>, but not the whole sequence of this part was covered. (<a href="href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF</a>, <a href=href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR</a>)</li>
+
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>alsS</i>.</li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>alsS</i></li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>alsS</i></li>
                     </ul>
                     </ul>
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                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilC</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilC</i></li>
-
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>ilvC</i>, but not the whole sequence of this part was covered. (<a href="href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF</a>, <a href=href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR</a>)</li>
+
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>ilvC</i>.</li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>ilvC</i></li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>ilvC</i></li>
                     </ul>
                     </ul>
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                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilvD</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilvD</i></li>
-
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>ilvD</i>, but not the whole sequence of this part was covered. (<a href="href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF</a>, <a href=href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR</a>)</li>
+
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>ilvD</i>.</li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>ilvD</i></li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>ilvD</i></li>
                     </ul>
                     </ul>
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                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>kivD</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>kivD</i></li>
-
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>kivD</i>, but not the whole sequence of this part was covered. (<a href="href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF</a>, <a href=href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR</a>)</li>
+
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Sequencing" target="_blank">Sequencing</a> confirmed the identity of pSB1C3-<i>kivD</i>.</li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>kivD</i></li>
                         <li>Glycerin stock created: <i>E. coli</i> KRX pSB1C3-<i>kivD</i></li>
                     </ul>
                     </ul>
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            <ul>
            <ul>
        <li>Annealing temperature: 55 &deg;C</li>
        <li>Annealing temperature: 55 &deg;C</li>
-
        <li>Bands not as expected (1,8 kb)</li>
+
        <li>Bands not as expected (~ 1800 bp)</li>
            </ul>
            </ul>
                   </ul>
                   </ul>
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            <ul>
            <ul>
        <li>Annealing temperature: 55 &deg;C</li>
        <li>Annealing temperature: 55 &deg;C</li>
-
        <li>Bands not as expected (1,55 kb)</li>
+
        <li>Bands not as expected (~ 1550 bp)</li>
            </ul>
            </ul>
                   </ul>
                   </ul>
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            <ul>
            <ul>
        <li>Annealing temperature: 55 &deg;C</li>
        <li>Annealing temperature: 55 &deg;C</li>
-
        <li>Bands not as expected (1,95 kb)</li>
+
        <li>Bands not as expected (~ 1950 bp)</li>
            </ul>
            </ul>
                   </ul>
                   </ul>
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            <ul>
            <ul>
        <li>Annealing temperature: 55 &deg;C</li>
        <li>Annealing temperature: 55 &deg;C</li>
-
        <li>Bands not as expected (1,75 kb)</li>
+
        <li>Bands not as expected (~ 1750 bp)</li>
            </ul>
            </ul>
                   </ul>
                   </ul>
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            <ul>
            <ul>
        <li>Annealing temperature: 55 &deg;C</li>
        <li>Annealing temperature: 55 &deg;C</li>
-
        <li>Bands not as expected (2,2 kb)</li>
+
        <li>Bands not as expected (~ 2.200 kb)</li>
            </ul>
            </ul>
                   </ul>
                   </ul>

Latest revision as of 09:12, 17 October 2014


June

  • alsS
    • This week we aim to transform the construct from the parts distribution.
  • ilvC
    • This week we aim to transform the construct from the parts distribution.
  • ilvD
    • This week we aim to transform the construct from the parts distribution.
  • kivD
    • This week we aim to transform the construct from the parts distribution.