Team:Aachen/Project/Measurement Device

From 2014.igem.org

(Difference between revisions)
(→WatsOn)
Line 1: Line 1:
__NOTOC__
__NOTOC__
 +
{{CSS/Main}}
 +
{{Team:Aachen/Stylesheet}}
{{Team:Aachen/Header}}
{{Team:Aachen/Header}}
Line 15: Line 17:
   <li>
   <li>
-
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fluorescence" style="color:black">
+
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonfunctions" style="color:black">
-
     <div class="menusmall-item menusmall-info" ><div class="menukachel">A Faster Answer</div></div>
+
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Functions</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
Line 23: Line 25:
   <li>
   <li>
-
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fret" style="color:black">
+
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurarty" style="color:black">
-
     <div class="menusmall-item menusmall-info" ><div class="menukachel">The FRET System</div></div>
+
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Measurarty</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
Line 31: Line 33:
   <li>
   <li>
-
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#darkquencher" style="color:black">
+
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurements" style="color:black">
-
     <div class="menusmall-item menusmall-info" ><div class="menukachel">REACh Quenchers</div></div>
+
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Measurements</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
Line 39: Line 41:
   <li>
   <li>
-
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#gfp-reach" style="color:black">
+
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonsecretingredients" style="color:black">
-
     <div class="menusmall-item menusmall-info" ><div class="menukachel">The Fusion Protein</div></div>
+
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Secret<br/>Ingredients</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     </div>
     </div>
Line 47: Line 49:
  <li>
  <li>
-
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#tevprotease" style="color:black">
+
     <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonachievements" style="color:black">
-
    <div class="menusmall-item menusmall-info" ><div class="menukachel">TEV Protease</div></div>
+
-
    <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/dc/Aachen_14-10-13_TEV_Protease_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
+
-
    </div>
+
-
    </a>
+
-
  </li>
+
-
 
+
-
<li>
+
-
    <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements" style="color:black">
+
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Achieve-<br/>ments</div></div>
     <div class="menusmall-item menusmall-info" ><div class="menukachel">Achieve-<br/>ments</div></div>
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/b/ba/Aachen_14-10-13_REACh_construct_tile_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
     <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/b/ba/Aachen_14-10-13_REACh_construct_tile_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%">
Line 65: Line 59:
</center>
</center>
</html>
</html>
 +
 +
{{Team:Aachen/BlockSeparator}}
 +
 +
== Functions ==
 +
<span class="anchor" id="watsonfunctions"></span>
''WatsOn'' fulfills several tasks in our system:
''WatsOn'' fulfills several tasks in our system:
Line 78: Line 77:
{{Team:Aachen/BlockSeparator}}
{{Team:Aachen/BlockSeparator}}
-
= Measurarty =
+
== Measurarty ==
 +
<span class="anchor" id="watsonmeasurarty"></span>
 +
''Awaiting synthesis...''
-
{{Team:Aachen/BlockSeparator}}
+
=== Software Evaluation ===
-
= Measurements =
+
''Awaiting synthesis...''
{{Team:Aachen/BlockSeparator}}
{{Team:Aachen/BlockSeparator}}
-
= Software Evaluation =
+
== Measurements ==
 +
<span class="anchor" id="watsonmeasurements"></span>
 +
 
 +
''Awaiting synthesis...''
{{Team:Aachen/BlockSeparator}}
{{Team:Aachen/BlockSeparator}}
-
= Secret Ingredients =
 
 +
== Secret Ingredients ==
 +
<span class="anchor" id="watsonsecretingredients"></span>
 +
 +
''Awaiting synthesis...''
[https://2014.igem.org/Template:Team:Aachen/arduino_device.ino?action=raw Arduino Sketchbook (.ino)]
[https://2014.igem.org/Template:Team:Aachen/arduino_device.ino?action=raw Arduino Sketchbook (.ino)]
{{Team:Aachen/BlockSeparator}}
{{Team:Aachen/BlockSeparator}}
-
= Build your own ''WatsOn'' =
+
== Achievements ==
 +
<span class="anchor" id="watsonachievements"></span>
 +
 
 +
''Awaiting synthesis...''
 +
 
 +
{{Team:Aachen/BlockSeparator}}
 +
 
 +
== Build your own ''WatsOn'' ==
 +
 
 +
---> Move to "Engineering"
 +
 
If you want to create your own ''WatsOn'' at first check the following list of necessary devices.
If you want to create your own ''WatsOn'' at first check the following list of necessary devices.

Revision as of 17:01, 14 October 2014

WatsOn

Aachen Device 11.jpg
'

Part of the biosensor system Cellock Holmes are our agar chips. They contain our engineered sensing cells that fluoresce in the presence of the pathogen Pseudomonas aeruginosa. However, bacteria are small and the fluorescent signal cannot be seen with the naked eye. Thus the central question "What's on the chip?" remains.

To answer this question we present our measurement device WatsOn.

Functions

WatsOn fulfills several tasks in our system:

  • incubate the sensing cells and the sampling chip
  • prevent escape of potentially sampled pathogens and our genetically engineered cells
  • illuminate the chip with the right excitation wavelength for GFP or iLOV
  • take photos and timeseries of the chips
  • analyze the fluorescence signal
  • give feedback to the user about the presence or absence of P. aeruginosa

WatsOn is designed such that it can be easily copied. Our work heavily emphasizes the Open Source concept. Therefore, the construction manual and all technical detailes are published on our wiki. Analogous to our OD/F device, we used low-cost and easily available parts.

Measurarty

Awaiting synthesis...

Software Evaluation

Awaiting synthesis...

Measurements

Awaiting synthesis...


Secret Ingredients

Awaiting synthesis... Arduino Sketchbook (.ino)

Achievements

Awaiting synthesis...

Build your own WatsOn

---> Move to "Engineering"

If you want to create your own WatsOn at first check the following list of necessary devices.

Table 1: Needed number of pieces, components and costs for building your own measurement device

number of pieces components costs [$]
1 filter slides (medium yellow 010, sally green 505)5.17
1 arduino UNO R311.65
1 2-channel relay shield3.46
40jumper-wire cable2.99
1 40er male header (10-Piece Pack)2.72
1 40er female header (10-Piece Pack)2.60
1 circuit board2.99
1 HDMI cable1.87
1 7 port USB hub6.71
1WLAN USB stick5.35
1USB mouse and keyboard12.50
1 case acrylic glass XT 6mm~0.5250.65
1spray paint for acrylic glass6.54
1 Raspberry Pi model B board35.00
1Raspberry Pi camera module25.00
17” display50.50
18-segment display3.86
2 digital temperature sensor DHT-227.50
1 aluminum block 100x100x15 mm14.23
1 Peltier heater 12V 60W4.49
1power supply32.89
6 superflux LED 480nm7.50
16LED 450nm7.50
1cupboard button1.24
-total308.55

You can find more economical information for WatsOn and the project on our Economical View page.

Aachen Device Fritzing.png
Wiring of our device
Aachen Device 1.jpg Start building your own WatsOn by assembling the base plate, the sides and the interior wall.
350px] [File:Aachen_Device_.3jpg Fix the Peltier heater on the back of the aluminum block and place it in the hole of the interior wall.
Arrange the 4x4 450nm LEDs and the 2x3 480nm LEDs
Aachen Device 7.jpg Assemble the camera holder with the camera and the corresponding filter slide on the lower part. Over the camera you can place the temperature sensor for measuring the indoor temperature. Finally put the fan on the back of the camera holder.
Aachen Device 8.jpg Connect the electronic components on the outside and the inside according to the wiring diagramm.
Aachen Device 4.jpg Put together the drawer.
Aachen Device 9.jpg Position the front panel and insert the drawer.
Aachen Device 10.jpg Place the temperature sensor for the aluminium block temperature directly on the block and put the back panel in front of it.
Aachen Device 6.jpg Setup the power supply and connect all devices to either 5V or 12V.
Aachen Device 11.jpg Mount the device on top of the power supply casing. Add the display and apply some stickers to enjoy your custom-made WatsOn.

Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Project/Measurement_Device"