Team:Aachen/Project/2D Biosensor
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{{Team:Aachen/Figure|Aachen_B0015_IPTG_HSL_Pseudomonas.png|title=Negativ control |subtitle=B0015 in NEB as negativ control induced with A) 0.2 µl of 100 mM IPTG, image taken after 2.5 h B) 0.2 µl of 500 µg/ml HSL (3-oxo-C12), image after 2.5 h C) with 5 spots of ''Pseudomonas aeruginosa'' on the left and one big spot on the right, image taken after 2h|width=900px}} | {{Team:Aachen/Figure|Aachen_B0015_IPTG_HSL_Pseudomonas.png|title=Negativ control |subtitle=B0015 in NEB as negativ control induced with A) 0.2 µl of 100 mM IPTG, image taken after 2.5 h B) 0.2 µl of 500 µg/ml HSL (3-oxo-C12), image after 2.5 h C) with 5 spots of ''Pseudomonas aeruginosa'' on the left and one big spot on the right, image taken after 2h|width=900px}} | ||
- | + | === Testing our Sensor Chips in a Platereader === | |
{{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | ||
+ | To establish a proof of principle. | ||
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{{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | ||
Detecting the 3-oxo-C{{sub|12}} HSL with K131026 in our sensor chip with WatsOn. | Detecting the 3-oxo-C{{sub|12}} HSL with K131026 in our sensor chip with WatsOn. |
Revision as of 08:48, 16 October 2014
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