Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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- | + | <!-- <li><a href="https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensormolecularapproach" style="color:black"> | |
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+ | </li> --> | ||
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+ | <li><a href="https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensordevelopments" style="color:black"> | ||
+ | <div class="team-item team-info" > | ||
+ | <br/><br/> | ||
+ | <b>Achievements</br> | ||
+ | <br/><br/> | ||
+ | click for more information | ||
+ | </div> | ||
+ | <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/e/ef/Aachen_14-10-15_Medal_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a> | ||
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- | + | <li><a href="https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements" style="color:black"> | |
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[[File:Aachen_14-10-14_cellock_stehend_panel.png|right|150px]] | [[File:Aachen_14-10-14_cellock_stehend_panel.png|right|150px]] | ||
- | == | + | == Development & Optimization Process == |
- | <span class="anchor" id=" | + | <span class="anchor" id="biosensordevelopment"></span> |
=== Medium === | === Medium === | ||
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(Zeitaufnahmen bearbeitet von Arne) | (Zeitaufnahmen bearbeitet von Arne) | ||
- | + | {{Team:Aachen/BlockSeparator}} --> | |
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+ | [[File:Aachen_14-10-14_cellock_stehend_panel.png|right|150px]] | ||
+ | == Achievements == | ||
+ | <span class="anchor" id="biosensorachievements"></span> | ||
+ | We are able to detect IPTG, 3-oxo-C{{sub|12}} HSL and ''Pseudomonas aeruginosa''. To proof that your sensor cells produce the flourescence signal and not something else we have B0015 in NEB as negativ control. | ||
- | + | === Testing our Sensor Chips with a Platereader === | |
{{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | ||
{{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} |
Revision as of 08:26, 16 October 2014
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