Team:Aachen/Project/2D Biosensor
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Inside ''WatsOn'', the chips are incubated at 37 °C and populations of microorganisms stuck on the sampling chip start to multiply. ''P. aeruginosa'' secrets an increasing number of quorum sensing molecules in growing cell colonies which are taken up by our sensor cells where they induce a fluorescence signal. In case of ''P. aeruginosa'', we focused on a quorum sensing molecule called N-3-oxo-dodecanoyl-L-homoserine lactone, or 3-oxo-C-12-HSL for short, while our sensor cells comprise of genetically modified ''E. coli'' cells which are able to elecit a '''fluorescent response to the autoinducers'''. How we incorporate this 3-oxo-C-12-HSL detection system into the sensor cells is explained in more detail in the [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh Construct] section. | Inside ''WatsOn'', the chips are incubated at 37 °C and populations of microorganisms stuck on the sampling chip start to multiply. ''P. aeruginosa'' secrets an increasing number of quorum sensing molecules in growing cell colonies which are taken up by our sensor cells where they induce a fluorescence signal. In case of ''P. aeruginosa'', we focused on a quorum sensing molecule called N-3-oxo-dodecanoyl-L-homoserine lactone, or 3-oxo-C-12-HSL for short, while our sensor cells comprise of genetically modified ''E. coli'' cells which are able to elecit a '''fluorescent response to the autoinducers'''. How we incorporate this 3-oxo-C-12-HSL detection system into the sensor cells is explained in more detail in the [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh Construct] section. | ||
- | During incubation the chips can be illuminated with blue light at any time, and a | + | During incubation the chips can be '''illuminated with blue light''' at any time, and a '''photo of the chips''' is taken. The '''software ''Measurarty''''' then analyzes any fluorescent signal. <!-- Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. --> |
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Revision as of 07:59, 16 October 2014
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