Team:Aachen/Project/2D Biosensor
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''Cellock Holmes'' is devised based upon a SynBio approach comprised of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and to generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal. | ''Cellock Holmes'' is devised based upon a SynBio approach comprised of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and to generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal. | ||
- | Our '''sensor cells are immobilized in agar chips'''. To make the chips, we mix the '' | + | Our '''sensor cells are immobilized in agar chips'''. To make the chips, we mix the sensing cells, also known as ''Cellocks'', with liquid LB agar. In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and ready to use. |
''Add: Flow chart of chip measurement'' | ''Add: Flow chart of chip measurement'' | ||
- | To detect ''P. aeruginosa'' cells, the chip is placed on a hard surface that is potentially contaminated with the pathogen. Subsequently, the chip is introduced into our measurement device where it is incubated at 37 °C. After a short time, the chip is illuminated with blue light. | + | To detect ''P. aeruginosa'' cells, the chip is placed on a hard surface that is potentially contaminated with the pathogen. Subsequently, the chip is introduced into our measurement device ''WatsOn'' where it is incubated at 37 °C. After a short time, the chip is illuminated with blue light. ''WatsOn'' takes a picture of the chip and the software ''Measurarty'' analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. |
Revision as of 15:01, 10 October 2014
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