Team:Aachen/Parts

From 2014.igem.org

(Difference between revisions)
(→human galectin-3, codon optimized for E. coli)
(→iGEM Team Aachen BioBricks)
Line 43: Line 43:
This part is a TEV protease in RFC25 that was optimized for expression in ''E. coli''. The part contains the S219V anti-self cleavage mutation.
This part is a TEV protease in RFC25 that was optimized for expression in ''E. coli''. The part contains the S219V anti-self cleavage mutation.
 +
 +
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==
 +
 +
=== IPTG-induced and T7-driven expression of TEV protease ===
 +
 +
This protein generator produces TEV protease when induced with IPTG in a DE3 strain.
 +
 +
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==
 +
 +
=== Constitutive expression of K1319000 ===
 +
 +
This part expresses K1319000 behind a J23101 constitutive promotor.
 +
 +
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==
 +
 +
=== Constitutive expression of K1319001 ===
 +
 +
This part expresses K1319001 behind a J23101 constitutive promotor.
 +
 +
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==
 +
 +
=== LasI induced iLOV ===
 +
 +
This device produces iLOV (K660004) in response to a quorum sensing input.
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==

Revision as of 14:08, 7 October 2014

iGEM Team Aachen BioBricks

[http://parts.igem.org/Part:BBa_K1319000 K1319000]

RFC [25] Version of E0020

This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.

[http://parts.igem.org/Part:BBa_K1319001 K1319001]

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Two mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W


[http://parts.igem.org/Part:BBa_K1319002 K1319002]

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W
  • H148R


[http://parts.igem.org/Part:BBa_K1319003 K1319003]

human galectin-3, codon optimized for E. coli

The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.

[http://parts.igem.org/Part:BBa_K1319004 K1319004]

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

[http://parts.igem.org/Part:BBa_K1319008 K1319008]

IPTG-induced and T7-driven expression of TEV protease

This protein generator produces TEV protease when induced with IPTG in a DE3 strain.

[http://parts.igem.org/Part:BBa_K1319010 K1319010]

Constitutive expression of K1319000

This part expresses K1319000 behind a J23101 constitutive promotor.

[http://parts.igem.org/Part:BBa_K1319011 K1319011]

Constitutive expression of K1319001

This part expresses K1319001 behind a J23101 constitutive promotor.

[http://parts.igem.org/Part:BBa_K1319017 K1319017]

LasI induced iLOV

This device produces iLOV (K660004) in response to a quorum sensing input.

[http://parts.igem.org/Part:BBa_K1319020 K1319020]

Translational unit of mRFP-galectin3-his

This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015).


[http://parts.igem.org/Part:BBa_K1319042 K1319042]

IPTG inducible iLOV

This part can be used for IPTG-induced expression of K660004 (iLOV).



  1. Part Name
  2. Part type
  3. Creator
  4. Sequence
  5. Short Description (60 characters on what the DNA does)
  6. Long Description (Longer description of what the DNA does)
  7. Design considerations


<groupparts>iGEM14 Aachen</groupparts>

Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Parts"