Team:Aachen/Parts

From 2014.igem.org

(Difference between revisions)
m (→IPTG inducible iLOV)
Line 7: Line 7:
===RFC [25] Version of E0020===
===RFC [25] Version of E0020===
-
This part is a RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.
+
This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.
==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
Line 13: Line 13:
===RFC [25] -compatible dark quencher based on K1319000 (E0030)===
===RFC [25] -compatible dark quencher based on K1319000 (E0030)===
-
This part is a RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
+
This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Two mutations were introduced that eliminated fluorescence:
Two mutations were introduced that eliminated fluorescence:
Line 24: Line 24:
===RFC [25] -compatible dark quencher based on K1319000 (E0030)===
===RFC [25] -compatible dark quencher based on K1319000 (E0030)===
-
This part is a RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
+
This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Three mutations were introduced that eliminated fluorescence:
Three mutations were introduced that eliminated fluorescence:
Line 36: Line 36:
===human galectin-3, codon optimized for ''E. coli''===
===human galectin-3, codon optimized for ''E. coli''===
-
Galectin-3 is a 26 kDa protein that binds certain LPS patterns.
+
Galectin-3 is a 26 kDa protein that binds certain LPS patterns.
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==

Revision as of 13:25, 6 October 2014

iGEM Team Aachen BioBricks

[http://parts.igem.org/Part:BBa_K1319000 K1319000]

RFC [25] Version of E0020

This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.

[http://parts.igem.org/Part:BBa_K1319001 K1319001]

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Two mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W


[http://parts.igem.org/Part:BBa_K1319002 K1319002]

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W
  • H148R


[http://parts.igem.org/Part:BBa_K1319003 K1319003]

human galectin-3, codon optimized for E. coli

Galectin-3 is a 26 kDa protein that binds certain LPS patterns.

[http://parts.igem.org/Part:BBa_K1319004 K1319004]

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

[http://parts.igem.org/Part:BBa_K1319020 K1319020]

Translational unit of mRFP-galectin3-his

This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015).


[http://parts.igem.org/Part:BBa_K1319042 K1319042]

IPTG inducible iLOV

This part can be used for IPTG-induced expression of K660004 (iLOV).



  1. Part Name
  2. Part type
  3. Creator
  4. Sequence
  5. Short Description (60 characters on what the DNA does)
  6. Long Description (Longer description of what the DNA does)
  7. Design considerations


<groupparts>iGEM14 Aachen</groupparts>

Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Parts"