Team:Aachen/Notebook/Wetlab/May
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AZimmermann (Talk | contribs) (Created page with "__NOTOC__ {{Team:Aachen/Header}} = May = == 1st == * gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M *: → 120 V, 30 min *: → cut out the bands == 5th...") |
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* 250 µl Chloramphilicol from a 35 mg/mL stock was added | * 250 µl Chloramphilicol from a 35 mg/mL stock was added | ||
* The cultures were grown until OD<sub>600</sub>= 0.6, then one was induced with IPTG | * The cultures were grown until OD<sub>600</sub>= 0.6, then one was induced with IPTG | ||
+ | <center> | ||
{| class="wikitable" | {| class="wikitable" | ||
! time !! colspan="3" | info/ OD | ! time !! colspan="3" | info/ OD | ||
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| '''14:11'''||A: 0.93 ||B: 0.91 ||C: 0.942 | | '''14:11'''||A: 0.93 ||B: 0.91 ||C: 0.942 | ||
|} | |} | ||
+ | </center> | ||
→ a negative control without plasmid was left out | → a negative control without plasmid was left out | ||
Revision as of 14:54, 8 October 2014
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