Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
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Week Twelve |
Sunday, 8/31/14
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Export Systems
Redo of cloning of agr system:
- Ran colony PCR on 10 picked overnight colonies.
- colony PCR products were then run on a gel.
- 5 LB-CARB liquid cultures were set up and incubated overnight.
De-FLAGging pTG005 for LC/MS analysis:
- Ran colony PCR on 10 picked overnight colonies.
- colony PCR products were then run on a gel.
- 3 LB-CARB liquid cultures were set up and incubated overnight.
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Monday, 9/1/14
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Export Systems
Redo of cloning of agr system:
- Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.
De-FLAGging pTG005 for LC/MS analysis:
- Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.
lamBCDA & fsrABC Reception Systems
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Tuesday, 9/2/14
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lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
- Ran colony PCR using liquid cultures of Gibson colonies inoculated on Friday, in hopes of obtaining clearer gel results
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Wednesday, 9/3/14
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Export Systems
- Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
- Ran gel of colony PCR products from yesterday
- Gel still yielded unfavorable results
- Planning to clone using Golden Gate assembly
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Thursday, 9/4/14
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Export Systems
- Sequencing data appears to confirm successful unFLAGging of pTG005.
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Friday, 9/5/14
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Saturday, 9/6/14
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