Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
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Week Ten |
Monday, 8/18/14
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Export Systems
Preparation of fsr system for LC/MS analysis:
- started vacufuging pAA001 acetonitrile samples (negative controls).
- Colony PCR was run on 8 more colonies picked from pTG005-noFLAG plate (RTH reaction run last week).
- Ran the PCR products on a gel.
- Set up 6 LB-CARB liquid cultures corresponding to the promising colonies, as determined by the colPCR
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
- Performed another QuickLigation reaction using yesterday's round-the-horn PCR product.
- Ligation reaction product was transformed into JM109.
- Picked six colonies from yesterday's transformations (RTH) and restreaked on a new plate & started overnight liquid cultures.
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Tuesday, 8/19/14
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Export Systems
- The 6 liquid cultures prepared yesterday were miniprepped and shipped for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
- Performed colony PCR on the round-the-horn colonies obtained from Sunday's & Monday's transformations.
- Sent promising colPCR products for sequencing.
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Wednesday, 8/20/14
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Preparation of our powerpoint presentations for SURF seminar day (see Thursday's entry).
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Thursday, 8/21/14
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Today is Summer Seminar Day (for SURF, our official summer research program)! All of us gave ~20-minute presentations on our individual subprojects.
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Friday, 8/22/14
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Break day.
Lab clean-up in preparation for transition into the post-research phase.
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