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Team:Bielefeld-CeBiTec/Results/DataPage
From 2014.igem.org
Data Page
This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our project part of the Wiki.
With our project we like to address the problem of energy storage as well as the increasing atmospheric concentration of carbon dioxide. Excess electricity will be used to bind atmospheric carbon dioxide in bacteria. The carbon is used to produce different commercially interesting products. As a proof of concept we decided to implement this system using Escherichia coli. Major advantages of our concept are the use of carbon dioxide from the atmosphere under usage of available electricity on the one hand and the production of isobutanol on the other.
Data for rMFC
| BioBrick | Description |
|---|---|
| BBa_K1465101 | Cytochrome c from Geobacter sulfurreducens for electron transfer with mediators for reduction equivalent generation |
| BBa_K1465102 | Fumerate reductase (frd) from E. coli under the control of the T7 promoter |
| BBa_K1465103 | Fumerate reductase (FumABCD) from Actinobacillus succinogenes |
| BBa_K1465104 | Fumarate reductase FumBCD from Actinobacillus succinogenes |
| BBa_K1465105 | Fumarate reductase (Fum) under control of the T7 promoter |
| BBa_K1465107 | Primer sequence for genome editing of porine fwd |
| BBa_K1465108 | Primer sequence for genome editing of porine rev |
| BBa_K1465109 | Primer sequence for genome editing of dcuB fwd |
| BBa_K1465110 | Primer sequence for genome editing of dcuB rev |
Data for CO2 Fixation
| BioBrick | Description |
|---|---|
| BBa_K1465201 | Phosphoribulokinase prkA from Synechoccus elongatus, codon optimized for E. coli. |
| BBa_K1465202 | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus |
| BBa_K1465203 | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechoccus elangatus. |
| BBa_K1465204 | Shell-associated protein (csoS2) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465205 | Carbonic anhydrase (csoS3) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465206 | shell proteins (csoS4) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465207 | pore-forming protein (csoS1D) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465208 | can + shell proteins (cloning intermediate) from Halothiobacillus neapolitanus |
| BBa_K1465209 | Carbonic anhydrase + shell proteins + csoS1D from Halothiobacillus neapolitanus |
| BBa_K1465212 | Phosphoribulokinase prkA from Synechoccus elongatus under the control of the ptac promoter |
| BBa_K1465213 | RubisCO from Halothiobacillus neapolitanus under control of the ptac promoter |
| BBa_K1465214 | Phosphoribulokinase of Halothiobacillus neapolitanus under control of ptac |
| BBa_K1465222 | shell-protein fused with GFP |
| BBa_K1465223 | Empty Carboxysome with GFP |
| BBa_K1465225 | Knock-down with sRNA of phosphofructo kinase. (sRNA:pfkA) |
| BBa_K1465226 | Knock-down with sRNA of phosphofructo kinase under control of ptac promoter (ptac_sRNA:pfkA) |
| BBa_K1465227 | Knock-down with sRNA of phosphofructo kinase under control of T7 promoter. (t7_sRNA:pfkA) |
| BBa_K1465228 | Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus |
| BBa_K1465229 | Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus under the control of the ptac promoter |
| BBa_K1465230 | Purification vector (T7-RBS Intein-tag) |
Data for Isobutanol
| BioBrick | Description |
|---|---|
| BBa_K1465301 | Alcohol dehydrogenase from Lactococcus lactis |
| BBa_K1465302 | Isobutanol producing pathway (alsS_ilvC_ilvD_kivD) |
| BBa_K1465303 | Isobutanol producing pathway combined with alcohol dehydrogenase (alsS_ilvC_ilvD_kivD_adhA) |
| BBa_K1465304 | Alcohol dehydrogenase under control of T7 promoter |
| BBa_K1465305 | Alcohol dehydrogenase under control of ptac promoter |
| BBa_K1465306 | Isobutanol producing pathway under control of ptac promoter |
| BBa_K1465307 | Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promoter |
Data for Biosafety
| BioBrick | Description |
|---|---|
| BBa_K1465401 | Alanin racemase (alr) knock-out strain fused with RFP as visual control of transformation in E.coli |
| BBa_K1465403 | Primer for alanin racemase (alr) fwd |
| BBa_K1465404 | Primer for alanin racemase (alr) rev |
| BBa_K1465405 | Primer for dadX fwd |
| BBa_K1465406 | Primer for dadX rev |
