Team:Hannover/Primer

From 2014.igem.org

Protocols / Primer

Here you find a list including the oligonucleotides used.

Name

Sequence in 5' → 3' Direction

01_pORE_E3_2x35S fwd

agcgtcggcgaggctaaaaagaacaaatgt

02_pORE_E3_2x35S rev

ggaaGGATCCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA

03_Oligo1_RCF[21]

GATCGAATTCAGATCTGGATCCtaaCTCGAG

04_Oligo2_RCF[21]

CGCGCTCGAGttaGGATCCAGATCTGAATTC

05_F1_gfp_EMP

AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTC

06_R1_gfp_EMP

CCGGACTTGTCATCGTCGTCCTTGTAGTCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG

07_R2_gfp_EMP

agcgtcggcgaggctaaaaagaacaaatgt

08_TMBP_pASK_fwd

ggaaGAATTCgGACGAtGATGAtAAGGTGGTTATCAACGGCGTCAAGT

09_TMBP_pASK_rev

ggaaCCATGGgtTTGTCgTCGTCaTCCTTACAGCTGCAAGATGATCCGC

10_Expa4 in pSB1C3 fwd

aaGAATTCGCGGCCGCTTCTAGatggctattaaactagcaattc

11_Expa4 in pSB1C3 rev

aaCTGCAGCGGCCGCTACTAGTAagcgtcggcgaggct

12_TMBP in pSB1C3 fwd

aaGAATTCGCGGCCGCTTCTAGATGGTGGTTATCAACGGCGTCAAGT

13_TMBP in pSB1C3 rev

aaCTGCAGCGGCCGCTACTAGTAttattaCTTACAGCTGCAAGATGATCCGC

14_CBD in pSB1C3 fwd

aaGAATTCGCGGCCGCTTCTAGATGACCAGTTCTATGTCAGTAGAATTTT

15_CBD in pSB1C3 rev

aaCTGCAGCGGCCGCTACTAGTAttattaaCGGCCTGACGCAAATCC

16_VF2

tgccacctgacgtctaagaa

17_VR

attaccgcctttgagtgagc

T7

CGACTCACTATAGGGCG

BackSeq-pGII

attaccggcctttgagtgagc