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Team:Hannover/Primer
From 2014.igem.org
Protocols / Primer
Here you find a list including the oligonucleotides used.
| Name | Sequence in 5' → 3' Direction |
| 01_pORE_E3_2x35S fwd | agcgtcggcgaggctaaaaagaacaaatgt |
| 02_pORE_E3_2x35S rev | ggaaGGATCCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA |
| 03_Oligo1_RCF[21] | GATCGAATTCAGATCTGGATCCtaaCTCGAG |
| 04_Oligo2_RCF[21] | CGCGCTCGAGttaGGATCCAGATCTGAATTC |
| 05_F1_gfp_EMP | AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTC |
| 06_R1_gfp_EMP | CCGGACTTGTCATCGTCGTCCTTGTAGTCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |
| 07_R2_gfp_EMP | agcgtcggcgaggctaaaaagaacaaatgt |
| 08_TMBP_pASK_fwd | ggaaGAATTCgGACGAtGATGAtAAGGTGGTTATCAACGGCGTCAAGT |
| 09_TMBP_pASK_rev | ggaaCCATGGgtTTGTCgTCGTCaTCCTTACAGCTGCAAGATGATCCGC |
| 10_Expa4 in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGatggctattaaactagcaattc |
| 11_Expa4 in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAagcgtcggcgaggct |
| 12_TMBP in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGATGGTGGTTATCAACGGCGTCAAGT |
| 13_TMBP in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAttattaCTTACAGCTGCAAGATGATCCGC |
| 14_CBD in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGATGACCAGTTCTATGTCAGTAGAATTTT |
| 15_CBD in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAttattaaCGGCCTGACGCAAATCC |
| 16_VF2 | tgccacctgacgtctaagaa |
| 17_VR | attaccgcctttgagtgagc |
| T7 | CGACTCACTATAGGGCG |
| BackSeq-pGII | attaccggcctttgagtgagc |
