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Team:TU Eindhoven/Notebook
From 2014.igem.org
Notebook
January
-Background research
February
-Background research
March
-Selection of topic
-Further research
-Selection of projec
April
-Further research
May
General Activities
Regular team meetings
Looking for sponsors
Month planning
June
Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX
July
Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR
Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX
Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)
Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA
Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa
August
Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve
Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent
Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis
Week 35: 25 August – 31 August
September
Wetlab
Week 36: 1 September – 7 September
Week 37: 8 September – 14 September
Week 38: 15 September – 21 September
Week 39: 22 September – 28 September
October
Wetlab
Week 40: 29 September – 5 October
Week 41: 6 October – 12 October
Week 42: 13 October – 19 October
