Team:TU Eindhoven/Project/protocols

From 2014.igem.org

Project Description

Protocols

Introduction

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

genetic engineering protocols

The following protocols are used in the Biolab during the modification of bacteria.

Colony PCR Antibody Titration with FACS

FACS for sorting with DBCO-TAMRA

FACS for sorting with DBCO-PEG(10kDa)

Vector Ligation

Overhang PCR

PCR purification of DNA fragments

Plasmid Amplification

Plasmid Gene Digestion

Plasmid Purification

Preparative Steps

Protein Expression Curve

Protein Expression

Site Directed Mutagenesis