Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Oct
From 2014.igem.org
October |
- Electrobiochemical reactor system and cultivation
- This week we continued to cultivate in the H-cell reactor by using a potentiostat to control and ensure a constant potential during the whole cultivation.
- Cultivation of KRX wildtype B0015 and KRX ΔdcuB::oprF with pSB1C3_T7_frd was planned
- To document the growth curve and the consumption of substrate daily samples were taken for OD600 measurement and HPLC analytics
- To evaluate the current consumption of the cells a chronoamperometric measurement was performed