Team:Caltech/week10

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Week 8

Week 9

Week 10

Week 11

Week 12

Week Ten

Monday, 8/18/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Performed another QuickLigation reaction using yesterday's round-the-horn PCR product.
  • Ligation reaction product was transformed into JM109.
  • Picked six colonies from yesterday's transformations (RTH) and restreaked on a new plate & started overnight liquid cultures.

Tuesday, 8/19/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Performed colony PCR on the round-the-horn colonies obtained from Sunday's & Monday's transformations.
  • Sent promising colPCR products for sequencing.

Wednesday, 8/20/14

 Preparation of our powerpoint presentations for SURF seminar day (see Thursday's entry).

Thursday, 8/21/14

 Today is Summer Seminar Day (for SURF, our official summer research program)! All of us gave ~20-minute presentations on our individual subprojects.

Friday, 8/22/14

 Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

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