Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Aug

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August

Week 1     08/04 - 08/10
Week 1     08/04 - 08/10
  • Promotors
    • We tried to assmeble some inserts with three different promotors to test which one is the best choice.
      • BioBrick Assembly (Suffix)
        • Backbones (digested with SpeI, PstI)
          • T7
          • pTac
          • pTet
        • Inserts (digested with XbaI, PstI)
          • prkA
          • Hneap
          • SELAN
          • sRNA:pfkA
      • Only the assembly with the pTac promotor was successful.
  • csoS1-4
    • We tried to [...]
      • Colony PCR (VF-Primer, VR-Primer)
      • Gibson Assembly
      • Plasmid isolation
      • Restriction digestion with SpeI and XbaI
        • Bands as expected()
  • glpX
    • We tried to [...]
      • Gibson Assembly after DpnI digestion
      • Colony PCR
  • prkA
    • We tried to [...]
      • PCR amplification and purification for insertion in pHnCBcsoS1D
        • Bands as expected ()
      • Gibson Assembly with pHnCBcsoS1D
      • Plasmid isolation of pTac_prkA

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