Team:TU Eindhoven/Notebook

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TU Eindhoven iGEM Team

Notebook

January

-Background research



February

-Background research



March

-Selection of topic
-Further research
-Selection of projec



April

-Further research



May

General Activities
Regular team meetings
Looking for sponsors
Month planning



June

Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX



July

Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX

Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)

Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa



August

Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve

Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent

Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis

Week 35: 25 August – 31 August



September

Wetlab
Week 36: 1 September – 7 September

Week 37: 8 September – 14 September

Week 38: 15 September – 21 September

Week 39: 22 September – 28 September



October

Wetlab
Week 40: 29 September – 5 October

Week 41: 6 October – 12 October

Week 42: 13 October – 19 October