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Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
Revision as of 10:13, 24 August 2014 by SebastianBlunk (Talk | contribs)
Primerlist
| Primer ID | Primer sequence |
|---|---|
| Module 1 | |
| Gene synthesis | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| CCM-Cassette (E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
| Cytochrom C (Geobacter sulfurreducens) | |
| pccH_fwd (55 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘ |
| pccH_rev (40 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘ |
| pSB1C3_pre_pccH (58 bp) | 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_pccH (43 bp) | 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| ??? (Julian) | |
| T7_frd_Ec_fwd (64 bp) | 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘ |
| T7_frd_Ec_rev (44 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘ |
| pSB1C3_frd_pre (45 bp) | 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘ |
| pSB1C3_frd_suf (47 bp) | 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pR6K_dcuB_short (22 bp) | 5‘-AATTAACCCTCACTAAAGGGCG-3‘ |
| Module 2 | |
| Carbonic anhydrase | |
| csoS3_can_fwd (50 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘ |
| pSB1C3_pre_can (46 bp) | 5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘ |
| csoS3_can_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘ |
| pSB1C3_suf_can (46 bp) | 5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| Shell associated protein | |
| csoS2_sap_1_fwd (43) | 5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘ |
| pSB1C3_pre_sap_1 (43 bp) | 5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| csoS2_sap_1_rev (38 bp) | 5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘ |
| csoS1D | |
| csoS1D_fwd (40 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘ |
| csoS1D_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘ |
| pSB1C3_suf_csoS1D (43bp) | 5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_csoS1D (43 bp) | 5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘ |
