• Miniprep ctxA, ctxB.
• Test digest and ran gel for ctxA, ctxB.
• Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
• Prepared for sequencing: diluted to the correct volume and concentration.
• Purified RBS (digested overnight yesterday).
• Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
• Inoculate more colonies from ctxA, ctxB plates.

RBS-Carb Gel was good.

Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.