Team:Evry/Notebook/Sensing/PCBs/week6
From 2014.igem.org
Week 6
Sensor construction hbpR-hbpC
Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
Sensor construction hbpR-hbpC
Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm