Team:Evry/Notebook/Sensing/PCBs/week6

From 2014.igem.org

Week 6

08/4/14 :

08/5/14 :

Sensor construction hbpR/PhbpC

Table 1: Received primers to check part BBa_K1155001 (Part given by Paris-Saclay team)

08/6/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C

08/7/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm

08/8/14 :

Part Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plasmid purification with NucleoSpin Plasmid (Macherey-Nagel) protocol: 487,9 ng/µL