Team:Caltech/Project/Details
From 2014.igem.org
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
Three quorum sensing systems were chosen to test in E. coli:
agrBCDAlamBCDAThe lamBCDA system is a quorum sensing system native to Lactobacillus plantarum that is homologous to the agrBCDA system mentioned above. lamB is purported to be a membrane protein assisting in the export and post-translational modification of lamD, the signaling peptide precursor, while lamC is the histidine kinase membrane receptor protein responsible for detecting presence of lamD and, upon doing so, phosphorylating lamA, the response regulator, which proceeds to activate transcription of any gene downstream of the pLam promoter [is that its name?].fsrABCThe fsrABC system is a quorum sensing system native to Enterococcus faecalis believed to regulate virulence factors involved in biofilm formation in its native host. In this system, FsrC and FsrA proteins function as histidine kinase receptor and response regulator respectively, while the FsrB protein is a membrane protein that is cleaved to form GBAP, the peptide mature signaling ligand. |