Team:Duke/Notebook
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Lab Notebook
April 2014 | ||||||
Month 1 of Project | ||||||
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May 2014 | ||||||
Month 2 of Project | ||||||
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June 2014 | ||||||
Month 3 of Project | ||||||
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July 2014 | ||||||
Month 4 of Project | ||||||
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August 2014 | ||||||
Month 5 of Project | ||||||
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31 |
September 2014 | ||||||
Month 6 of Project | ||||||
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October 2014 | ||||||
Month 7 of Project | ||||||
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April Overview
Objectives
This is what we were trying to accomplish in April. For more detail, Click Here .
May 29
Objective: Prepare Chemically competent E. Coli
Matthew Faw
Followed Charlie’s Cloning Protocols to prepare chemically competent E. Coli
- Once E. Coli were prepared, the solution was aliquoted into 12 labeled tubes (450l in each tube) and tubes were stored in iGEM box in -80C cooler on far left
Next Steps:
- Transformation
June 1
Objective: Transform Chemically competent E. Coli (CCEC)
Matthew Faw
Transformation of CCEC with RFP Construct of varying concentrations+control with no DNA
- Followed Charlie’s Cloning Protocols
- Mistakenly used all of DNA construct from the kit… So not sure if the transformation will be successful
- Plated the transformed DNA and put in incubator at 37C
- Results (6/2/14)--Transformation unsuccessful because improper procedure was done by MFaw
Next Steps:
- Look at plates, compare cultures
June 2
Objective: Redo yesterday’s transformation
Matthew Faw
Due to my failure to correctly conduct the transformation yesterday, it was necessary to redo the transformation.
- Followed Charlie’s Cloning Protocols
- Added 1 ul of .5, 5, 10, 20, 50, and 0 (control) pg/lof RFP Construct to chemically competent E. Coli (CCEC), and followed procedure
- Plated the transformed DNA on 6 separate plates, put in 37C overnight
- Results: (6/3/14)-Transformation unsuccessful. Try the transformation again with Charlie’s cells and with new cells grown using iGEM’s protocol
Next Steps:
- Look at plates, compare cultures, etc.