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From 2014.igem.org

Contents 1 Friday 11th July 1.1 Lab work 1.1.1 1 - Fill in the WIKI (Notebook and Protocols) 1.1.2 2 - Preparation of competent cells E.coli 1.1.3 3 - Electrophoresis 1.1.4 3 - Bacterial Culture

Friday 11th July

Lab work

1 - Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

2 - Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

• DH5α
• MG1655Z1

Sean did the first day, Maher did the second one.

Protocol

3 - Electrophoresis

by Fabio & Marie

1. Cm FNR-RBS-AmilCP term (1)
2. Cm FNR-RBS-AmilCP term (2)
3. B0015 (1)
4. B0015 (2)
5. PCola-GES 10^-2 (1)
6. PCola-GES 10^-2 (1)
7. PCola-GES 10^-2 (2)
8. PCola-GES 10^-2 (2)
9. PJBG-6409 10^-2 (1)
10. PJBG-6409 10^-2 (2)
11. PJBG-6409 10^-2 (2)

Protocol

3 - Bacterial Culture

by Mathieu

Strains used: J23106 and J23114 from the concentrated colony made the 10th July

5uL of antibiotic in each tube.

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