Team:HokkaidoU Japan/Notebook/asB0034/Ptet B0034 32 asB0034 assay

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Notebook

Lab Documents

• Top
• RNA in Love
  • Overview
  • Background
  • Achievements

  • H-stem System
  • Overview
  • Stabilization
  • Modelling
  • Construct
  • How To Use

  • Anti-sense B0034
  • Overview
  • Method
  • Results
  • Conclusion

  • Length Variation
  • Overview
  • Method
  • Results
  • Conclusion

  • Extra Materials
  • Parts
  • Notebook
  • Safety
• Outreach
  • Univ. Festival
  • Education
  • Survey

  • High School
  • Education
  • Survey

  • Discussion
  • Background
  • Evaluation
• About Us
  • About Us
  • Acknowledgements
  • Team

P_tet-B0034,B0032,-mRFP-dT
P_tet-B0032-mRFP-dT&asB0034Assay

• Start

• Preculture

  • Sun Sep 14

  R0040-B0034-E1010-B0015 (on pSB6A1)

  R0040-B0032-E1010-B0015 (on pSB6A1)

  Ptrc-stem-asB0034-stem(on pHN1257)

  R0040-B0032-E1010-B0015 (on pSB6A1)

  Turbidity was controlled 0.1

• Culture

  • Mon Sep 15

  M9ZB 2 mL IPTG 20 µ or not for 9 hours,

  11 hours, 13hours

• Assay

  • Tue Sep 16

  fluoresence imageing 514 nm 610 filter

• Complete!

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