Team:Evry/Notebook/Sensing/PCBs/09-05-2014

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Picture

Sensor construction bphR2/PbpR1

Miniprep was made for each part and the nanodrop gives:

  • J23114-rbs: 205 ng/µl
  • bphR2: 227,5ng/µl
  • bphR1:57,2ng/µl
  • RFP: 74,2ng/µl
  • terminator:54,6ng/µl
  • pSB1C3,G3: 182,7ng/µl


A new PCR has been done by using Q5 DNA polymerase for all the parts expected for the terminator (one taq polymerase)

Sep 05

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