"
Team:Evry/Notebook/Sensing/PCBs/09-05-2014
From 2014.igem.org
Sensor construction bphR2/PbpR1
Miniprep was made for each part and the nanodrop gives:
- J23114-rbs: 205 ng/µl
- bphR2: 227,5ng/µl
- bphR1:57,2ng/µl
- RFP: 74,2ng/µl
- terminator:54,6ng/µl
- pSB1C3,G3: 182,7ng/µl
A new PCR has been done by using Q5 DNA polymerase for all the parts expected for the terminator (one taq polymerase)
Sep 05
