Team:Bordeaux/NotebookAugust28

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28th August

Molecular biology

  • PCR on our CFU

    Buffer 5X GC 10μl
    Phusion 0,5μl
    dNTP 1μl
    primer reverse (10mM) 2,5μl
    primer forward (10mM) 2,5μl
    H2O qsp 50μl


    =>Gel 1% (30min, 50V)

  • Resolubilisation of ELP at 1mg/ml for hysteresis

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