From 2014.igem.org

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28^th August

• PCR on our CFU
  
  

  Buffer 5X GC	10μl
  Phusion	0,5μl
  dNTP	1μl
  primer reverse (10mM)	2,5μl
  primer forward (10mM)	2,5μl
  H2O	qsp 50μl

  
  
  =>Gel 1% (30min, 50V)


• Resolubilisation of ELP at 1mg/ml for hysteresis