NOTEBOOK

Materials:

1.Control Unit

• 1.Arduino UNO

2.Power Supply

• 1.Computer Power Supply Unit (ATX)
• 2.Custom designed plate | 2.1. 5V and 12V conectors

3.Electric Syringe

• 1.Stteper motor
• 2.Endless worm
• 3.Custom designed parts
• 4.Syringe
• 5.IV extension tubes
• 6.3-Way Stopcock

4.Robotic Arm

• 1.Servo motors
• 2.Custom designed parts

5.Centrifuge

• 1.Brushless motor
• 2.Esc for motor
• 3.Centrifuge arm (custom designed)
• 4.Motor support (custom designed)
• 5.PCR tube support (custom designed)
• 2.Esc for motor

1.Done manually:

• 1.Collect a 2 mL blood sample from patient in EDTA tube with 2 mL of RNA later;
• 2.Take a 500 μL fraction from the collected blood sample and transfer to 1.5mL PCR tubes;

2.Robot:

• 1.Adds 1mL of lysis solution (custom designed) in the 500 μL of blood, mix and wait 10 minutes;
• 2.After 10 minutes, heat the PCR tube at 56°C for 10 minutes;
• 3.Centrifuge at 8.000 rpm for 5 minutes;
• 4.Heat the PCR tube at 98ºC for 1min;
• 1.Take 5 μL of the supernatant part of the liquid inside the PCR tube to the biosensor.